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Following my first degree in Biochemistry at the University of Oxford (Merton College), I moved to the Department of Engineering Science and Linacre College to pursue a PhD/DPhil in molecular microbiology and biochemistry. During this time I developed an interest in DNA replication and repair, studying thermoacidophilic archaea and their DNA polymerases. To pursue my interests in genomics, I then undertook a Masters degree in Bioinformatics at the University of York with a research placement at the University of Uppsala in Sweden.
I subsequently moved into eukaryotic molecular biology, working for 3 years at the Nuffield Department of Clinical Laboratory Sciences in Oxford. Here I researched microRNA and cancer-testis antigen expression profiles in human lymphomas, cementing my interests in cancer research. I then undertook two postdoctoral positions at the Structural Genomics Consortium (Oxford), working on high-throughput crystallography and biochemistry of human DNA polymerases and helicases involved in genome integrity, SNF2 family members in chromatin remodelling and ETS transcription factors.
I continued to research genome integrity factors in cancer during a short postdoc at the Sir William Dunn School of Pathology (Oxford), using structural, biochemical and cell biology approaches to study endonucleases and chromatin remodeling SNF2 ATPases involved in the DNA damage response. During my latter postdoc positions I was appointed as an E.P. Abraham Cephalosporin Junior Research Fellow and a member of the Governing Body at Linacre College, and also as a College Lecturer in Biochemistry at the Queen’s College.
I joined the University of Huddersfield as a Senior Lecturer in Biological Sciences in 2015.
My main focus is to take a multi-disciplinary approach to study proteins involved in the DNA damage response and genome integrity in human cancers, using structural biochemistry and biophysics, molecular cell biology and bioinformatics methodologies. From my previous work I am also interested in the biology of ETS transcription factors and Cancer-Testis antigens. Understanding the basic functions of these molecules at the molecular level will shed light on their roles in cancer development and metastasis, giving the potential to develop novel biomarkers or chemotherapeutic treatments. I am a member of CeBioR (Centre for Biomarker Research) and I receive funding from Kidscan Children’s Cancer Research.
I also have interests in developing novel protein expression and purification systems to facilitate structural and biochemical approaches, and also the biotechnological exploitation of novel DNA metabolising proteins from extremophilic bacteria and archaea.
Sebesta, M., Cooper, C., Ariza, A., Carnie, C. and Ahel, D. (2017) ‘Structural insights into the function of ZRANB3 in replication stress response’ Nature Communications , 8 (15847). ISSN 2041-1723
Cooper, C. and Marsden, B. (2017) ‘N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools.’. In: Heterologous Gene Expression in E.coli: Methods and Protocols. : Springer. pp. 11-31. ISBN 978-1493968879
Newman, J., Cooper, C., Roos, A., Aitkenhead, H., Oppermann, U., Cho, H., Osman, R. and Gileadi, O. (2016) ‘Structures of Two Melanoma-Associated Antigens Suggest Allosteric Regulation of Effector Binding’ PLoS ONE , 11 (2), pp. 1-20. ISSN 1932-6203
Newman, J., Cooper, C., Aitkenhead, H. and Gileadi, O. (2015) ‘Structure of the Helicase Domain of DNA Polymerase Theta Reveals a Possible Role in the Microhomology-Mediated End-Joining Pathway’ Structure , 23 (12), pp. 2319-2330. ISSN 0969-2126
Cooper, C., Newman, J., Aitkenhead, H., Allerston, C. and Gileadi, O. (2015) ‘Structures of the Ets Protein DNA-binding Domains of Transcription Factors Etv1, Etv4, Etv5, and Fev: Determinants of DNA Binding and Redox Regulation by Disulfide Bond Formation.’ The Journal of Biological Chemistry , 290 (22), pp. 13692-13709. ISSN 1083-351X
Newman, J., Cooper, C., Aitkenhead, H. and Gileadi, O. (2015) ‘Structural insights into the autoregulation and cooperativity of the human transcription factor Ets-2’ The Journal of Biological Chemistry , 290 (13), pp. 8539-8549. ISSN 1083-351X
Cooper, C., Newman, J. and Gileadi, O. (2014) ‘Recent advances in the structural molecular biology of Ets transcription factors: interactions, interfaces and inhibition’ Biochemical Society Transactions , 42 (1), pp. 130-138. ISSN 0300-5127 (print), 1470-8752 (electronic)
Canning, P., Cooper, C., Krojer, T., Murray, J., Pike, A., Chaikuad, A., Keates, T., Thangaratnarajah, C., Hojzan, V., Ayinampudi, V., Marsden, B., Gileadi, O., Knapp, S., von Delft, F. and Bullock, A. (2013) ‘Structural basis for Cul3 protein assembly with the BTB-Kelch family of E3 ubiquitin ligases’ The Journal of Biological Chemistry , 288 (11), pp. 7803-7814. ISSN 1083-351X
Twigg, S., Lloyd, D., Jenkins, D., El�ioglu, N., Cooper, C., Al-Sannaa, N., Annag�r, A., Gillessen-Kaesbach, G., H�ning, I., Knight, S., Goodship, J., Keavney, B., Beales, P., Gileadi, O., McGowan, S. and Wilkie, A. (2012) ‘Mutations in multidomain protein MEGF8 identify a Carpenter syndrome subtype associated with defective lateralization’ American Journal of Human Genetics , 91 (5), pp. 897-905. ISSN 1537-6605
Keates, T., Cooper, C., Savitsky, P., Allerston, C., Phillips, C., Hammarstr�m, M., Daga, N., Berridge, G., Mahajan, P., Burgess-Brown, N., M�ller, S., Gr�slund, S. and Gileadi, O. (2012) ‘Expressing the human proteome for affinity proteomics: optimising expression of soluble protein domains and in vivo biotinylation’ New Biotechnology , 29 (5), pp. 515-525. ISSN 1876-4347
Cooper, C., Lawrie, C., Liggins, A., Collins, G., Hatton, C., Pulford, K. and Banham, A. (2011) ‘Identification and characterization of peripheral T-cell lymphoma-associated SEREX antigens’ PLoS ONE , 6 (8), p. e23916. ISSN 1932-6203
Lucic, B., Zhang, Y., King, O., Mendoza-Maldonado, R., Berti, M., Niesen, F., Burgess-Brown, N., Pike, A., Cooper, C., Gileadi, O. and Vindigni, A. (2011) ‘A prominent ?-hairpin structure in the winged-helix domain of RECQ1 is required for DNA unwinding and oligomer formation’ Nucleic Acids Research , 39 (5), pp. 1703-1717. ISSN 0305-1048
Savitsky, P., Bray, J., Cooper, C., Marsden, B., Mahajan, P., Burgess-Brown, N. and Gileadi, O. (2010) ‘High-throughput production of human proteins for crystallization: the SGC experience’ Journal of Structural Biology , 172 (1), pp. 3-13. ISSN 1095-8657
Yue, W., Hozjan, V., Ge, W., Loenarz, C., Cooper, C., Schofield, C., Kavanagh, K., Oppermann, U. and McDonough, M. (2010) ‘Crystal structure of the PHF8 Jumonji domain, an Nepsilon-methyl lysine demethylase’ FEBS Letters , 584 (4), pp. 825-830. ISSN 0014-5793
Loenarz, C., Ge, W., Coleman, M., Rose, N., Cooper, C., Klose, R., Ratcliffe, P. and Schofield, C. (2010) ‘PHF8, a gene associated with cleft lip/palate and mental retardation, encodes for an Nepsilon-dimethyl lysine demethylase’ Human Molecular Genetics , 19 (2), pp. 217-222. ISSN 0964-6906
Lawrie, C., Cooper, C., Ballabio, E., Chi, J., Tramonti, D. and Hatton, C. (2009) ‘Aberrant expression of microRNA biosynthetic pathway components is a common feature of haematological malignancy’ British Journal of Haematology , 145 (4), pp. 545-548. ISSN 1365-2141
Lawrie, C., Saunders, N., Soneji, S., Palazzo, S., Dunlop, H., Cooper, C., Brown, P., Troussard, X., Mossafa, H., Enver, T., Pezzella, F., Boultwood, J., Wainscoat, J. and Hatton, C. (2008) ‘MicroRNA expression in lymphocyte development and malignancy’ Leukemia , 22 (7), pp. 1440-1446. ISSN 1476-5551
Lawrie, C., Soneji, S., Marafioti, T., Cooper, C., Palazzo, S., Paterson, J., Cattan, H., Enver, T., Mager, R., Boultwood, J., Wainscoat, J. and Hatton, C. (2007) ‘MicroRNA expression distinguishes between germinal center B cell-like and activated B cell-like subtypes of diffuse large B cell lymphoma’ International Journal of Cancer , 121 (5), pp. 1156-1161. ISSN 0020-7136
Liggins, A., Cooper, C., Lawrie, C., Brown, P., Collins, G., Hatton, C., Pulford, K. and Banham, A. (2007) ‘MORC4, a novel member of the MORC family, is highly expressed in a subset of diffuse large B-cell lymphomas’ British Journal of Haematology , 138 (4), pp. 479-486. ISSN 0007-1048
Sahota, S., Goonewardena, C., Cooper, C., Liggins, A., Ait-Tahar, K., Zojer, N., Stevenson, F., Banham, A. and Pulford, K. (2006) ‘PASD1 is a potential multiple myeloma-associated antigen’ Blood , 108 (12), pp. 3953-3955. ISSN 0006-4971
Cooper, C., Liggins, A., Ait-Tahar, K., Roncador, G., Banham, A. and Pulford, K. (2006) ‘PASD1, a DLBCL-associated cancer testis antigen and candidate for lymphoma immunotherapy’ Leukemia , 20 (12), pp. 2172-2174. ISSN 0887-6924
Funded PhD studentships will be advertised when available, but prospective Masters or PhD students who are self-funded (e.g. through government sponsorship or scholarships) are welcome to approach me informally by email in the first instance.
Current research projects include:
I am module leader for SFB1010 (Research Skills) and SIB2003 (Molecular Biology), teach on SFB1008 (World of Microbes), examine on SHB4003 (Applied Molecular Genetics), and offer research projects for SHB4001/SHB4014 modules. I am also the SWE Placement tutor (SIB2999) for third year placements and the staff liason for HUBS (Huddersfield University Bioscience Society).