Portrait of Dr Andrew Collett Dr Andrew Collett

View research degree topics that Dr Andrew Collett might supervise

a.collett@hud.ac.uk | 01484 473587



Biography

My first degree was in Applied Biology at the University of Hatfield I then went on to do a PhD at the University of Newcastle upon Tyne researching the mechanism of novel epithelial chloride channel blockers. I then worked at the University of Manchester in the Departments of Medicine and Pharmacy investigating intestinal drug absorption using in vitro models. After that I moved to Dundee University where my main research interest was ion transport across the lung epithelia. I returned to work in the Department of Pharmacy at Manchester and continued my interest in intestinal drug absorption and the role of P-glycoprotein. Most recently I have worked in the Department of Translational Medicine in Manchester investigating intestinal inflammation and the importance of the interaction between P-glycoprotein and the enteric micro-flora.

I was appointed as a Lecturer in Biological Sciences at Huddersfield in 2008 my main teaching is Cellular and Molecular Biology to MPharm students.

Research and Scholarship

I have two major areas of research, firstly an ongoing interest in the development of more predictive in vitro models of intestinal drug absorption particularly in the area of controlled release preparations and the potential modifying role of intestinal transport proteins.

In addition I also conduct research in the area of intestinal inflammation particularly the interaction of the gut epithelial cells with the normal enteric microflora. This is tightly regulated in health but can be the source of significant pathology in susceptible individuals leading to chronic inflammatory conditions such as Crohn's disease and Ulcerative colitis. My work tries to understand at a molecular level the mechanisms that initiate the inflammatory response in intestinal epithelial cells.

Publications and Other Research Outputs

2017

Dunnill, C., Al-Tameemi, W., Collett, A., Haslam, I. and Georgopoulos, N. (2017) ‘A Clinical and Biological Guide for Understanding Chemotherapy-Induced Alopecia and its PreventionThe Oncologist . ISSN 1083-7159

Patten, D., Hussein, E., Davies, S., Humphreys, P. and Collett, A. (2017) ‘Commensal-derived OMVs elicit a mild proinflammatory response in intestinal epithelial cellsMicrobiology , 163 (5), pp. 702-711. ISSN 1465-2080

2014

Patten, D., Leivers, S., Chadha, M., Maqsood, M., Humphreys, P., Laws, A. and Collett, A. (2014) ‘The structure and immunomodulatory activity on intestinal epithelial cells of the EPSs isolated from Lactobacillus helveticus sp. Rosyjski and Lactobacillus acidophilus sp. 5e2.Carbohydrate Research , 384, pp. 119-127. ISSN 00086215

2013

Patten, D. and Collett, A. (2013) ‘Exploring the immunomodulatory potential of microbial-associated molecular patterns derived from the enteric bacterial microbiotaMicrobiology , 159 (8), pp. 1535-1544. ISSN 1465-2080

2009

Bannon, C., Davies, P., Collett, A. and Warhurst, G. (2009) ‘Potentiation of flagellin responses in gut epithelial cells by interferon-? is associated with STAT-independent regulation of MyD88 expressionBiochemical Journal , 423 (1), pp. 119-128. ISSN 0264-6021

2008

Collett, A., Higgs, N., Meritxell, G., Zeef, L., Hayes, A., Salmo, E., Haboubi, N., Iovanna, J., Carlson, G. and Warhurst, G. (2008) ‘Early molecular and functional changes in colonic epithelium that precede increased gut permeability during colitis development in mdr1a(?/?) miceInflammatory Bowel Diseases , 14 (5), pp. 620-631. ISSN 10780998

Collett, A (2008) ‘Investigation of Regional Mechanisms Responsible for Poor Oral Absorption in Humans of a Modified Release Preparation of the  -Adrenoreceptor Antagonist, 4-Amino-6,7-dimethoxy-2-(5-methanesulfonamido-1,2,3,4 tetrahydroisoquinol-2-yl)-5-(2-pyridyl)quinazoline (UK-338,003): The Rational Use of ex Vivo Intestine to Predict in Vivo AbsorptionDrug Metabolism and Disposition , 36 (1), pp. 87-94. ISSN 00909556

2005

Collett, A., Tanianis-Hughes, J., Carlson, G., Harwood, M. and Warhurst, G. (2005) ‘Comparison of P-glycoprotein-mediated drug–digoxin interactions in Caco-2 with human and rodent intestine: Relevance to in vivo predictionEuropean Journal of Pharmaceutical Sciences , 26 (5), pp. 386-393. ISSN 0928-0987

2004

Collett, A., Tanianis-Hughes, J. and Warhurst, G. (2004) ‘Rapid induction of P-glycoprotein expression by high permeability compounds in colonic cells in vitro: a possible source of transporter mediated drug interactions?Biochemical Pharmacology , 68 (4), pp. 783-790. ISSN 0006-2952

Collett, A., Tanianis-Hughes, J., Hallifax, D. and Warhurst, G. (2004) ‘Predicting P-Glycoprotein Effects on Oral Absorption: Correlation of Transport in Caco-2 with Drug Pharmacokinetics in Wild-Type and mdr1a(-/-) Mice in VivoPharmaceutical Research , 21 (5), pp. 819-826. ISSN 0724-8741

2002

Collett, A., Ramminger, S., Olver, R. and Wilson, S. (2002) ‘Beta-Adrenoceptor-mediated control of apical membrane conductive properties in fetal distal lung epitheliaAmerican journal of physiology - Lung cellular and molecular physiology , 282 (4), p. L621-L630. ISSN 1040-0605

2001

Baines, D., Ramminger, S., Collett, A., Haddad, J., Best, O., Land, S., Olver, R. and Wilson, S. (2001) ‘Oxygen-evoked Na+ transport in rat fetal distal lung epithelial cellsThe Journal of Physiology , 532 (1), pp. 105-113. ISSN 0022-3751

Haddad, J., Collett, A., Land, S., Olver, R. and Wilson, S. (2001) ‘NF-?B Blockade Reduces the O2-Evoked Rise in Na+ Conductance in Fetal Alveolar CellsBiochemical and Biophysical Research Communications , 281 (4), pp. 987-992. ISSN 0006-291X

Land, S. and Collett, A. (2001) ‘Detection of Cl- flux in the apical microenvironment of cultured foetal distal lung epithelial cellsJournal of Experimental Biology , 204 (4), pp. 785-795. ISSN 0022-0949

2000

McAlroy, H., Ahmed, S., Day, S., Baines, D., Wong, H., Yip, C., Ko, W., Wilson, S. and Collett, A. (2000) ‘Multiple P2Y receptor subtypes in the apical membranes of polarized epithelial cellsBritish Journal of Pharmacology , 131 (8), pp. 1651-1658. ISSN 0007-1188

1999

Inglis, S., Collett, A., McAlroy, H., Wilson, S. and Olver, R. (1999) ‘Effect of luminal nucleotides on Cl - secretion and Na + absorption in distal bronchiPflügers Archiv - European Journal of Physiology , 438 (5), pp. 621-627. ISSN 0031-6768

Ramminger, S., Collett, A., Baines, D., Murphie, H., McAlroy, H., Olver, R., Inglis, S. and Wilson, S. (1999) ‘P2Y2 receptor-mediated inhibition of ion transport in distal lung epithelial cellsBritish Journal of Pharmacology , 128 (2), pp. 293-300. ISSN 0007-1188

Collett, A., Higgs, N., Sims, E., Rowland, M. and Warhurst, G. (1999) ‘Modulation of the Permeability of H2 Receptor Antagonists Cimetidine and Ranitidine by P-Glycoprotein in Rat Intestine and the Human Colonic Cell Line Caco-21The Journal of pharmacology and experimental therapeutics , 288 (1), pp. 178-178. ISSN 0022-3565

Research Degree Supervision

The interaction of novel biologically active compounds with cellular drug efflux transporters

Projects in this area will test for potential interactions of newly synthesised biologically active compounds with efflux proteins. This important because in order for drugs to be most effective therapeutically, particularly in the central nervous system, it is advantages if they do not have significant interactions with cellular efflux transporters.

The role of microbial biofilms in the modulation of intestinal epithelial function

It is becoming increasingly apparent that the normal function of the epithelial cells that line the intestinal mucosa is dependent on their close interaction with the vast and complex micro-flora that resides in the intestine. Most research in this area has concentrated on the effects of individual bacterial species. This project will investigate the role of biofilms which are more realistic of the conditions found in the native intestine.

Translocation of bacteria across epithelial barriers

A vital function of the bodies epithelial barriers is to prevent the access of bacteria into the body thus translocation of bacteria across epithelial cells, particularly those that line the intestine can cause serious disease or death. Projects in this area will investigate the molecular mechanisms by which various bacterial species cross the intestinal epithelia and examine the role of the normal intestinal microflora in preventing this process occurring.

Postgraduate research opportunities with Dr Andrew Collett

2017-18

PhD

2018-19

PhD